Burgdorferi Antibody Igm Immunoblot

Burgdorferi Antibody Igm Immunoblot. Immunoblot testing is appropriate for confirming a detected EIA or IFA test result. However consideration must be given to possible contamination by blood or transfer of serum antibodies. ImmunoBlot strip is a membrane strip with fixed B.

Western Blot Analysis Of Specific Anti Borrelia Burgdorferi Antibodies Download Scientific Diagram

At the recommended lower cut-off value of.

Burgdorferi antibody is consistent with the presence of antibody produced by patients in response to infection by B. Burgdorferi and suggests the presence of Lyme. IgM antibody results against B burgdorferi should only be considered during the 30 days following exposure and symptom onset.

Additional specimens should be submitted in 2 to 3 weeks if B burgdorferi exposure has not been ruled out. Although the test has been shown to have a high degree of reliability for diagnostic purposes laboratory data should always be correlated with clinical findings. Immunoblot testing qualitatively examines with high specificity antibodies in a patients specimen.

Both IgM and IgG immunoblots should be performed on specimens less than 4 weeks after appearance of erythema migrans. Lyme Disease Antibodies IgG IgM Immunoblot - Lyme disease is transmitted by a tick vector carrying Borrelia burgdorferi. Burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to the P37 antigen.

The 37-kDa protein P37 of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M IgM antibody response in Lyme disease patients. A negative result indicates that the immunoblot evaluation for B. This test should be used for confirmation of an equivocal or positive B.

Burgdorferi antibody demonstrates no antibodies unique to B. The P37 gene was cloned from a B. Only IgG immunoblot should be performed on specimens greater than 4 weeks after disease onset.

Burgdorferi infection when sera are positive by IgM immunoblot and negative by IgG Western blot after the initial 4 week period from onset of symptoms. There was no difference in the bands detected by Lyme disease immunoblot with the sera collected at 1 3 and 6 weeks data not shown. In addition it is possible that some of the IgM immunoblot results were misclassified as false positive.

Western Blot Analysis Of Specific Anti Borrelia Burgdorferi Antibodies Download Scientific Diagram

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